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The use of an enzacryl AA derivative for affinity chromatography of sex hormone binding globulin

✍ Scribed by R. Hampl; O. Táborský; J. Musil; L. Stárka

Publisher: Elsevier Science
Year: 1974
Tongue: English
Weight: 577 KB
Volume: 97
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(01)97584-3

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✦ Synopsis

A polymer of the polyacrylamide type with side-chains bearing free amino groups on the benzene ring (Enzacryl AA) with covalently attached 3-hemisuccinate of 5u-androstane-3x.

17&dinl WLLS used for theaffinity chromatogr:lphy of ses hormone

binding $obulin_

After washing with buik of increasin 8 ionic strength, the release of protein was ttchievcd by treatment with a butTered solution of testosterone.

An spprosimately 63-fold enrichment of the required protein was achieved.

The purified protein was characterized by its binding properties and by isoelectric focusing_ --5p

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## Abstract Familial influences were investigated for baseline sex hormone‐binding globulin (SHBG) and its response (post‐training minus baseline) to a 20‐week endurance exercise training program. One hundred, eighty‐four participants from 85 Black families in the HERITAGE Family Study (HERITAGE) w