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The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes

✍ Scribed by Gary H. Perdew; Henry W. Schaup; Daniel P. Selivonchick

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
293 KB
Volume
135
Category
Article
ISSN
0003-2697

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✦ Synopsis

CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-Propane sulfonate, a zwitterionic detergent, has been shown to exhibit superior membrane protein solubilizing characteristics as compared to nonionic detergents. Replacement of NP-40 with CHAPS in isoelectric focusing of rainbow trout liver microsomes has increased resolution markedly. The two-dimensional electrophoretic system described will allow effective resolution of up to 300 micrograms of crude microsomal protein. CHAPS exhibits no effect on the stability or type of pH gradient when compared to NP-40 during isoelectric focusing in the presence of urea.

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