We report the effects of a strong overexpression of the GAL4 activator protein on the expression of UASGAL regulated genes, obtained by cloning the GAL4 gene and the GALl-10 upstream activating sequence (UAS~AL)-lacZ fusion in the same high copy number plasmid. Comparable amounts of active enzyme we
✦ LIBER ✦
The use of a ‘universal’ yeast expression vector to produce an antigenic protein of Mycobacterium leprae
✍ Scribed by Roger J. Booth; Prudence M. Grandison; Ross L. Prestidge; James D. Watson
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 399 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0165-2478
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## Abstract ## Background Transfecting cells with plasmid DNA encoding the protein Bax causes programmed cell death (apoptosis) and results in the formation of cell fragments (apoptotic bodies). It has been known for some time that when dendritic cells phagocytose apoptotic bodies derived from tum