The use of a CCD imaging luminometer in the quantitation of luminogenic immunoassays

✍ Scribed by Leaback, D. H. ;Haggart, R.

• Book ID: 102762037
• Publisher: John Wiley and Sons
• Year: 1989
• Weight: 530 KB
• Volume: 4
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170040167

✦ Synopsis

We describe the application of a CCD Imaging Luminometer (CCDIL) for the detection and quantitation of rapid, simultaneous, peroxidase-linked chemiluminescent immunoassays of multiple samples of human serum alphafetoprotein (AFP). Results from some analogous immunoassays (total IgE, and thyroid stimulating hormone (TSH)) are included for comparison.

Values for precision and antigen concentration obtained using the CCDIL and colorimetric versions of the immunoassays, on human serum samples, were in good agreement.

The flexibility of the CCDIL is demonstrated; its ability t o detect and quantitate antigen (particularly AFP) on a variety of solid phases is indicated.

The work on the AFP immunoassays illustrates not only the flexibility of the CCDIL for sample presentation on a variety of solid phase systems, but also some relative merits of such systems.