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The use of 2,2′-thiodiethanol in the fluorimetric determination of secondary amines with o-phthalaldehyde and 2-mercaptoethanol

✍ Scribed by A. Himuro; H. Nakamura; Z. Tamura

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
532 KB
Volume
147
Category
Article
ISSN
0003-2670

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✦ Synopsis

The fluorimetric determination of secondary amines based on conversion with sodium hypochlorite to primary amines and the o-phthalaldehyde-mercaptoethanol (OPA) reagent is discussed. Interferences by excess of hypochlorite (e.g., oxidation of OPA or conversion of primary amines to chloramines) were suppressed by 2,2'-thiodiethanol. Two spectrophotofluorimetric methods are reported, one for aromatic and the other for aliphatic secondary amines. These methods permitted determinations at nanomolar levels; relative standard deviations were 4% for 5 nmol of N-methylaniline and 13% for 5 nmol of sarcosine.

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