For much of the Middle and all of the Upper Pleistocene the Upper Thames valley has remained outside the limit of ice advance. The main agents of landform evolution have been the River Thames and its tributaries, which have cut down episodically and in so doing have abandoned a series of river terraces. This study reports the findings of an investigation into exposures in the deposits underlying the Floodplain Terrace at Cassington, near Oxford, England. The sequence exposed reveals a stratigraphy of basal, predominantly fine-grained, lithofacies overlain by coarser gravel lithofacies. The fluvial architecture of these deposits indicates a major change in fluvial style from a low-energy (meandering) to a high energy (braided) channel system. The flora and fauna from the lower fine-grained lithofacies display a marked change from temperate at the base, to colder conditions towards the top, indicating a close association between deteriorating climate and changing fluvial depositional style. Amino acid and luminescence geochronology from the basal fine-grained lithofacies suggest correlation with Oxygen Isotope Stage 5 and hence it is argued that the major environmental change recorded at the site relates to the Oxygen Isotope Stage 5-4 transition. Deposition of much of the overlying gravel sequence probably occurred during Oxygen Isotope Stage 4, suggesting that the latter half of the Devensian may be less significant, in terms of fluvial landscape evolution in the Upper Thames valley, than was believed previously.