The type II decoy receptor of IL-1 inhibits murine collagen-induced arthritis

✍ Scribed by Natacha Bessis; Laurence Guéry; Alberto Mantovani; Annunciata Vecchi; John E. Sims; Didier Fradelizi; Marie-Christophe Boissier

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 129 KB
• Volume: 30
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/1521-4141(200003)30:3<867::aid-immu867>3.0.co;2-m

✦ Synopsis

IL-1 is a key cytokine involved in the inflammatory response. The type II receptor of IL-1 (IL-1RII) acts as a decoy receptor, binding and inhibiting the effect of IL-1. This study was undertaken to establish whether IL-1RII can ameliorate collagen-induced arthritis, a model of inflammatory arthritis in mice. We used human keratinocytes transfected with the human (h)IL-1RII gene as a source of hIL-1RII protein. We showed that these cells expressed both the membrane and soluble form of receptor. In vitro, IL-1-stimulated murine macrophage cells showed a decreased expression of TNF- § in the presence of hIL-1RII. We engrafted the hIL-1RII-transfected cells in the back of mice developing collagen-induced arthritis. We found that clinical and histological parameters of arthritis were significantly decreased in mice treated with cells producing hIL-1RII. In addition, hIL-1RII administration was able to reduce the expression of mRNA for IL-6 and myeloperoxidase in the joints of treated animals. These data show that hIL-1RII anti-inflammatory properties in the model of collageninduced arthritis in mice and could have a regulatory role in rheumatoid arthritis.