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The transfer of triazine resistance fromBrassica napusL. toB. oleraceaL. III. First backcross to parental species

โœ Scribed by R. Ayotte; P. M. Harney; V. Souza Machado


Publisher
Springer
Year
1988
Tongue
English
Weight
357 KB
Volume
38
Category
Article
ISSN
0014-2336

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โœฆ Synopsis


Atrazine resistant Brassica hapus x B . oleracea F, hybrids were backcrossed to both parental species . The backcrosses to B. napus produced seeds in both directions but results were much better when the F, hybrid was the pollen parent . Backcrosses to B . oleracea failed completely but BC,s were rescued by embryo culture both from a tetraploid hybrid (2n = 4x = 37 ; A,C,CC) and sesquidiploid hybrids (2n = 3x = 28 ; A,C,C) .

Progeny of crosses between the tetraploid hybrid and B . oleracea had between 25 and 28 chromosomes . That of crosses between the sesquidiploid hybrid and B . oleracea had between 21 and 27 . A few plants that had chromosome counts outside the expected range may have originated from either diploid parthenogenesis, unreduced gametes or spontaneous chromosome doubling during in vitro culture . Pollen stainability of the BC,s ranged from 0% to 91 .5% . All the BC,s to B . oleracea were resistant to atrazine .


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The transfer of triazine resistance from
โœ R. Ayotte; P. M. Harney; V. Souza Machado ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 723 KB

Triazine resistant Brassica napus ssp. oleifera and ssp. rapifera were hybridized to cultivars of B. oleracea ssp. italica, ssp. botrytis, ssp. capitata and ssp.jimbriata. The interspecific embryos did not survive in vivo but could be rescued in vitro using a culture medium developed by MONNIER (19

The transfer of triazine resistance from
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F, hybrids of triazine resistant Brassica napus and triazine susceptible B . oleracea were morphologically intermediate to the parent species . Of 49 hybrids examined, 44 had 28 chromosomes, two had 37, one had 38 and two had 56 . The 38-chromosome plant was thought to be a matromorph, the others, A