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The transcriptional response of virulence genes in Listeria monocytogenes during inactivation by nisin

✍ Scribed by Shi, Hui; Trinh, Quoclinh; Xu, Wentao; Luo, Yunbo; Tian, Wenying; Huang, Kunlun

Book ID
121744447
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
476 KB
Volume
31
Category
Article
ISSN
0956-7135

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Gene disruption by plasmid integration in Listeria monocytogenes: Insertional inactivation of the listeriolysin determinant IisA
✍ Wuenscher, Michael D. ;KΓΆhler, Stephan ;Goebel, Werner ;Chakraborty, Trinad πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 782 KB

A plasmid integration technique was developed for insertional inactivation of chromosomal Listeria monocytogenes genes. A Listeria-Escherichia coli shuttle vector (pLSV1) was constructed which carried the temperature-sensitive gram-positive replication origin from plasmid pTV32(Ts). An internal frag