The transcription factor KLF11 can induce γ-globin gene expression in the setting of in vivo adult erythropoiesis

✍ Scribed by David W. Emery; Georgios Gavriilidis; Haruhiko Asano; George Stamatoyannopoulos

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 254 KB
• Volume: 100
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.21093

✦ Synopsis

Abstract

Previous studies in a fetal erythroid cell line demonstrated that the transcription factor, Krüppel‐like factor 11 (KLF11), could specifically induce transcription from a γ‐globin gene promoter, and that this induction was mediated through a specific canonical CACCC cis‐DNA binding motif. We report here that ectopic expression of KLF11 can also induce fetal γ‐globin gene expression in the setting of adult erythropoiesis both in vitro and in vivo. Studies in an adult‐stage murine erythroleukemia (MEL) cell line demonstrated that retrovirus vector‐mediated transduction of KLF11 could increase both the amount of expression from a basally active, but not from a overtly silenced, recombinant γ‐globin transgene, as well as the frequency of cells expressing this transgene. A similar pattern of γ‐globin gene induction was also observed both in vitro and in vivo following KLF11 transduction of bone marrow from mice containing a basally active γ‐globin transgene. These studies provide the first evidence that ectopic expression of a transcription factor can induce γ‐globin gene expression in vivo during adult erythropoiesis. J. Cell. Biochem. 100: 1045–1055, 2007. © 2006 Wiley‐Liss, Inc.