The Total Chemical Synthesis of Monocyte Chemotactic Protein-1 (MCP-1)

✍ Scribed by Angus R. Brown; Maryanne Covington; Robert C. Newton; Robert Ramage; Patricia Welch

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 576 KB
• Volume: 2
• Category: Article
• ISSN: 1075-2617
• DOI: 10.1002/psc.46.o

✦ Synopsis

Abstract

The affinity‐based N^α^‐amino protecting group tetrabenzo [a,c,g,i]fluorenyl‐17‐methoxycarbonyl (Tbfmoc) has been utilized as a hydrophobic probe to allow the simple, quick and highly effective isolation of a 76 residue cysteine‐containing protein (MCP‐1). The base‐labile Tbfmoc group can be removed under very mild conditions, which preserve the thiol‐con taining protein in the reduced state. Oxidative folding was then used to furnish the biologically active β‐chemokine MCP‐1.