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The topography of some anionic sites at the surfaces of fixed ehrlich ascites tumour cells

✍ Scribed by L. Weiss; O. S. Jung; R. Zeigel

Publisher
John Wiley and Sons
Year
1972
Tongue
French
Weight
960 KB
Volume
9
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The distribution of positively charged, colloidal iron hydroxide particles to the surfaces of glutaraldehyde‐fixed Ehrlich ascites tumour cells, was studied in 700Å thick sections. After incubation with neuraminidase and/or ribonuclease, and fixation, cells were also reacted with particles.

It was shown that the numbers of bound particles were significantly reduced by enzyme treatment, which had previously been shown to reduce the densities of anionic sites at the cell surfaces. Statistical analyses showed that in the controls and ribonuclease‐treated cells, the particles were adsorbed in surface locations following a Poisson distribution. Following neuraminidase treatment, the particles were arranged in clusters, but the clusters themselves were arranged in a Poisson distribution.

The data are tentatively interpreted as indicating the presence of different sized neuraminidase‐ and ribonuclease‐susceptible zones at the cell surface, having a higher than average density of anionic sites.

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The binding of cationized ferritin at th
The binding of cationized ferritin at the surfaces of ehrlich ascites tumor cells: The effect of pH and glutaraldehyde fixation
✍ J. R. Subjeck; L. Weiss 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 English ⚖ 771 KB

## Abstract The densities of cationized ferritin (CF) particles binding to the surfaces of cultured Ehrlich ascites tumor cells were determined at pH 7.4, where the ferritin stain was applied either prior to or following glutaraldehyde fixation. The densities were also determined with CF adjusted t