The tissue-specific patterns of lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), and G-6-phosphate dehydrogenase (G-6-PD) in adults and their occurrence during ontogeny of the guppy,Poecilia reticulata Peters (pisces: Poeciliidae)
✍ Scribed by Chingjiang, Wu ;Schröder, Johannes Horst
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 603 KB
- Volume
- 229
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Using polyacrylamide gel electrophoresis, the tissue-specific and ontogenetic patterns of three dehydrogenase enzymes (LDH, ADH, and G-6-PD) of the guppy were investigated. Only one band of G-6-PD was detected in extracts of the liver. Isozyme LDH-B4 was ubiquitous; isozyme LDH-A4 was detected predominantly in extracts of muscle, and isozyme LDH-C was only observed in extracts of the eye. Isozyme ADH-A was detected in all tissues except the heart, while isozymes ADH-B and ADH-C were only detected in extracts of the eye. Isozymes LDH-C and its heteromers with LDH-B first appear following complete differentiation of the retinal cells. The appearance of isozyme LDH-A coincided with the onset of contractile movements of muscle. Isozymes ADH-B and ADH-C are not detected until fry are 16 mm long after the onset of the complete differentiation of the retinal cells and visional function. Isozymes ADH-D and G-6-PD are enzymes found only in embryonic stages and suddenly disappear after hatching. They may be regulative enzymes of cell differentiation.