The time-course analysis of gene expression during wound healing in mouse skin

✍ Scribed by Shinichiro Kagawa; Aya Matsuo; Yoichi Yagi; Kazuya Ikematsu; Ryouichi Tsuda; Ichiro Nakasono

• Publisher: Elsevier Science
• Year: 2009
• Tongue: English
• Weight: 209 KB
• Volume: 11
• Category: Article
• ISSN: 1344-6223
• DOI: 10.1016/j.legalmed.2008.09.004

✦ Synopsis

RNA analysis has been applied to forensic work to determine wound age. We investigated mRNA expression using quantitative RT-PCR of ten genes, including c-fos, fosB, mitogen-activated protein kinase phosphatase-1 (MKP-1), CD14, chemokine (C-C motif) ligand 9 (CCL9), placenta growth factor (PlGF), mast cell protease-5 (MCP-5), growth arrest specific 5 (Gas5), beta-2 microglobulin (B2M) and major urinary protein-1 (MUP-1), in terms of repair response in adult mice. The expression level of c-fos, fosB and MKP-1 transcripts increased drastically, peaked within 1h, and that of the CD14 and CCL9 transcripts peaked from 12 to 24h. An increase in PlGF and MCP-5 mRNA appeared on about day 5. Gas5, B2M and MUP-1 transcripts showed no significant change. Each gene had differentially expressional patterns with time-course. Our result implied that the observation of the 7 genes in wounded skin could serve to aid in the accurate diagnosis of wound age.