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The Thulium Complex of 1,4,7,10-Tetrakis{[N-(1H-imidazol-2-yl)carbamoyl]methyl}-1,4,7,10-tetraazacyclododecane (dotami) as a ParaCEST Contrast Agent

✍ Scribed by Dirk Burdinski; Johan Lub; Jeroen A. Pikkemaat; Sander Langereis; Holger Grüll; Wolter ten Hoeve


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
294 KB
Volume
5
Category
Article
ISSN
1612-1872

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✦ Synopsis


Abstract

1,4,7,10‐Tetrakis{[N‐(1__H__‐imidazol‐2‐yl)carbamoyl]methyl}‐1,4,7,10‐tetraazacyclododecane (dotami), a tetra(1__H__‐imidazol‐2‐yl) derivative of the well‐studied octadentate 1,4,7,10‐tetrakis[(carbamoyl)methyl]‐1,4,7,10‐tetraazacyclododecane (dotam) ligand, was synthesized by reaction of 1,4,7,10‐tetraazacyclododecane with N‐(1__H__‐imidazol‐2‐yl)chloroacetamide in high yield. Its tricationic thulium complex was isolated as a water‐soluble chloride salt. The detection of the mildly acidic amide and amine protons by direct proton NMR in aqueous solution was unsuccessful, but such exchangeable protons could be detected via their chemical exchange‐dependent saturation transfer (CEST) effect. The observed CEST effect was distinctly different from that found for respective dotam complexes and is, therefore, ascribed to exchangeable protons associated with the imidazole substituent.