The teratogenic potential and dose–response of dermally administered ethylene glycol monomethyl ether (EGME) estimated in rats with the chernoff–kavlock assay
✍ Scribed by G. A. De S. Wickramaratne
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 162 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0260-437X
No coin nor oath required. For personal study only.
✦ Synopsis
The Chernoff and Kavlock in vivo assay was used to study the teratogenic potential of ethylene glycol monomethyl ether (EGME). It was administered to pregnant rats on days 6-17 (inclusive) of gestation by the dermal route at an application rate of 10 ml kg-' body weight of 3%, lo%, 30% and 100% EGME with physiological saline as the solvent. 100% was lethal to the pregnant rats, 30% was lethal to the fetuses, 10% produced small litter sizes with poor survival and 3% had no adverse effects. By the 'rules' developed to evaluate results from the assay, 10% was judged to be a potentially teratogenic dose in the rats. The study also demonstrated the usefulness of this assay for pre-screening or additional investigations for suspected teratogens when route of exposure could be important in determining the potential hazard.