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The synthesis of peptide-oligonucleotide conjugates by a fragment coupling approach

✍ Scribed by Suzanne Peyrottes; Béatrice Mestre; Fabienne Burlina; Michael J. Gait

Publisher
Elsevier Science
Year
1998
Tongue
French
Weight
646 KB
Volume
54
Category
Article
ISSN
0040-4020

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✦ Synopsis

Solid-phase synthesis of several peptide-oligonuclcotide conjugates has been achieved using a peptide fragment coupling strategy on a conlrolled pore glass support. The conjugates contain either a hydrophobic tetrapeptide LGIG or an 8-residue basic pcptide of the HIV-1 Tat protein coupled to one of two oligodeoxyribonucleotides, an oligoribonucleotide or a mixed ribo/2'-O-methyl oligonucleotide. Improved yields were obtained when internucleotide [I-cyanocthyl groups were removed from the support-bound oligonucleotide prior to pcptide fragment coupling, and by use of a long alkyl spacer in the linkage between peptide and oligonuclcotide.

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