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The synthesis of a photolabile detergent and its use in the isolation and characterization of protein

โœ Scribed by W.W. Epstein; D.S. Jones; E. Bruenger; H.C. Rilling

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
696 KB
Volume
119
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A new ionic detergent sodium 4-( 3,3-dimethyl-1 -oxotridecyl)benzenesulfonate similar to sodium dodecyl sulfate has been synthesized which is photodegradable to sodium p-acetylbenzenesulfonate and a simple olefin mixture upon irradiation in aqueous solution with light 300 nm and above in wavelength. This photodegradable detergent can be used to solubilize many proteins and provide information as to the molecular weight and subunit composition of proteins by electrophoresis. The removal of this detergent by photolysis results in no apparent damage to protein.

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