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The synthesis and use of oligodeoxynucleotides in plasmid DNA sequencing

✍ Scribed by Sarbjit S. Ner; David P. Bloxham; Penelope A. Handford; Muhammad Akhtar

Book ID
103118970
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
818 KB
Volume
18
Category
Article
ISSN
0020-711X

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A simple procedure for the precise assembly of functional DNA sequences from overlapping fragments is described. The fragments to be joined are cloned in tandem in the proper relative orientation into a vector from which single-stranded DNA copies can be obtained. Single-stranded DNA is cut by a res