The Surfaceome: Methods and Protocols

✍ Scribed by Kenneth R. Boheler,Rebekah L. Gundry (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 351
• Series: Methods in Molecular Biology 1722
• Edition: 1
• Category: Library

✦ Synopsis

This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.

✦ Table of Contents

Front Matter ....Pages i-xi
Front Matter ....Pages 1-1
Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis Antigens (Yulanda M. Williamson, Jennifer Whitmon, Rolieria West-Deadwyler, Hercules Moura, Adrian R. Woolfitt, Jon Rees et al.)....Pages 3-20
“Shaving” Live Bacterial Cells with Proteases for Proteomic Analysis of Surface Proteins (Manuel J. Rodríguez-Ortega)....Pages 21-29
Methods for Mapping the Extracellular and Membrane Proteome in the Avian Embryo, and Identification of Putative Vascular Targets or Endothelial Genes (Witold W. Kilarski, John Herbert, Andreas Bikfalvi)....Pages 31-56
Mass Spectrometry-Based Identification of Extracellular Domains of Cell Surface N-Glycoproteins: Defining the Accessible Surfaceome for Immunophenotyping Stem Cells and Their Derivatives (Chelsea M. Fujinaka, Matthew Waas, Rebekah L. Gundry)....Pages 57-78
Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and Its Proteome from Cells in Suspension (Rebecca L. Rose, Waeowalee Choksawangkarn, Catherine Fenselau)....Pages 79-90
Proteomic Profiling of Secreted Proteins, Exosomes, and Microvesicles in Cell Culture Conditioned Media (Ankit Sinha, Simona Principe, Javier Alfaro, Alex Ignatchenko, Vladimir Ignatchenko, Thomas Kislinger)....Pages 91-102
Front Matter ....Pages 103-103
Cloning, Expression, and Purification of the Glycosylated Transmembrane Protein, Cation-Dependent Mannose 6-Phosphate Receptor, from Sf9 Cells Using the Baculovirus System (Linda J. Olson, Nancy M. Dahms)....Pages 105-116
Bispecific Antibody Armed T Cells to Target Cancer Cells (Archana Thakur, Lawrence G. Lum, Sandeep Mittal)....Pages 117-126
Immunophenotyping of Live Human Pluripotent Stem Cells by Flow Cytometry (Daniel R. Riordon, Kenneth R. Boheler)....Pages 127-149
Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4 (Amanda M. Nevins, Adriano Marchese)....Pages 151-164
Front Matter ....Pages 165-165
NaV Channels: Assaying Biosynthesis, Trafficking, Function (Gordon F. Tomaselli, Federica Farinelli)....Pages 167-184
High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs) (Chi-Wing Kong, Lin Geng, Ronald A. Li)....Pages 185-194
Methods for Evaluation of Vascular Endothelial Cell Function with Transient Receptor Potential (TRP) Channel Drugs (Yung Wui Tjong, Xiaoqiang Yao)....Pages 195-210
Methods to Study the Signal Transduction of the Surface Receptor Tyrosine Kinase TrkB in Neurons (Kwok-On Lai, Nancy Y. Ip)....Pages 211-222
Polarized Human Retinal Pigment Epithelium Exhibits Distinct Surface Proteome on Apical and Basal Plasma Membranes (Vladimir Khristov, Qin Wan, Ruchi Sharma, Mostafa Lotfi, Arvydas Maminishkis, Kapil Bharti)....Pages 223-247
Extracellular Matrix Molecule-Based Capture of Mesenchymal Stromal Cells Under Flow (Teresa Massam-Wu, Stuart A. Cain, Cay M. Kielty)....Pages 249-260
Generation of Induced Pluripotent Stem Cells from Patients with COL3A1 Mutations and Differentiation to Smooth Muscle Cells for ECM-Surfaceome Analyses (Jiaozi He, Zhihui Weng, Stanley Chun Ming Wu, Kenneth R. Boheler)....Pages 261-302
Fabrication and Mechanical Properties Measurements of 3D Microtissues for the Study of Cell–Matrix Interactions (Prasenjit Bose, Chen Yu Huang, Jeroen Eyckmans, Christopher S. Chen, Daniel H. Reich)....Pages 303-328
Front Matter ....Pages 329-329
Discovery of Surface Target Proteins Linking Drugs, Molecular Markers, Gene Regulation, Protein Networks, and Disease by Using a Web-Based Platform Targets-search (Bin Yan, Panwen Wang, Junwen Wang, Kenneth R. Boheler)....Pages 331-344
Back Matter ....Pages 345-347

✦ Subjects

Cell Biology