A simple electrophoretic method for the
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Romilio T. Espejo; Jacob Lebowitz
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Article
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1976
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Elsevier Science
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English
β 590 KB
A simple method for directly dete~in~ng the superhelix density of closed circular DNAs by ethidium bromide titration using agarose ge1 electrophoresis in tubes containing increasing concentrations of the dye has been deveIoped. Since the positively charged dye migrates toward the cathode in a direct