✦ LIBER ✦

The subunits of the parasporal crystal ofBacillus thuringiensis: Size, linkage and toxicity

✍ Scribed by Hans E. Huber; Peter Lüthy; Hans-Rudolf Ebersold; Jean-Louis Cordier

Publisher: Springer
Year: 1981
Tongue: English
Weight: 837 KB
Volume: 129
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00417171

No coin nor oath required. For personal study only.

✦ Synopsis

The solubility properties and the number of disulfide groups per molecule of the crystal protein of Bacillus thuringiensis were shown to be similar to that of wool keratin. Dissolution of the crystals required scission of S-S bonds. This could be achieved at neutral p H without loss of toxicity. Molecular weight determinations with gel electrophoresis and ultracentrifugation indicated that the crystal subunit is a dimer. With the exception of the variety israelensis, all strains belonging to ten different subspecies revealed crystal subunits of the same molecular weight.

📜 SIMILAR VOLUMES

A rapid and simple method for staining o

A rapid and simple method for staining of the crystal protein ofBacillus thuringiensis

✍ Fadel A. Sharif; N. Gürdal Alaeddinoĝlu 📂 Article 📅 1988 🏛 Springer-Verlag 🌐 English ⚖ 796 KB

Isolation and assay of the toxic compone

Isolation and assay of the toxic component from the crystals ofBacillus thuringiensisvar.israelensis

✍ Elizabeth W. Davidson; Takashi Yamamoto 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 351 KB

Bacillus thuringiensis insecticidal delt

Bacillus thuringiensis insecticidal delta-endotoxin: Diversity of crystal proteins and its relatedness to the toxicity spectrum

✍ Dr. M. Z. Haider; S. Mahmood 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 499 KB

Bacillus rhuringiensis strains produce crystal delta-endotoxins which exhibit a diverse toxicity spectrum. In order to explore the basis of toxin specificity, a comparison of the activity of 13 strains belonging to seven serotypes was made against three insect species. The delta-endotoxin crystals w