The substrate specificity of tRNA (m1G37) methyltransferase (TrmD) from Aquifex aeolicus
โ Scribed by Hiroshi Takeda; Takashi Toyooka; Yoshiho Ikeuchi; Shin-ichi Yokobori; Kan Okadome; Fuyumi Takano; Tairo Oshima; Tsutomu Suzuki; Yaeta Endo; Hiroyuki Hori
- Book ID
- 108731894
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 401 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1356-9597
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The trmD gene, which governs the formation of 1-methyl-guanosine(m1G) in transfer ribonucleic acid (tRNA), has been located by phage P1 transduction at 56 min on the chromosomal map of Escherichia coli. Cotransduction to tyrA at 56 min is 80%. From the Clarke and Carbon collection a ColE1-tyrA+ hybr
The trmD gene, which is the structural gene for the tRNA(m1G)-methyltransferase, is shown to be part of a polycistronic operon. A 4.6 kb SalI-EcoRI chromosomal DNA fragment contains the trmD gene (Bystrรถm and Bjรถrk 1982). Subclonings, deletion mapping and Tn5 insertions into plasmid pBY03 have estab