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The submitochondrial distribution of acid: CoA ligase (AMP) and palmityl-CoA: Carnitine palmityltransferase in rat liver mitochondria

โœ Scribed by Kaare R. Norum; Mikael Farstad; Jon Bremer

Book ID
118849639
Publisher
Elsevier Science
Year
1966
Tongue
English
Weight
432 KB
Volume
24
Category
Article
ISSN
0006-291X

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A radiolabeled ATP assay was developed for measuring carboxylic acid:CoA ligase activity. The assay was designed to measure the formation of [gamma-33P]pyrophosphate from [gamma-33P]ATP in the course of the reaction. The assay was linear with protein concentration, and rates as low as 1 pmol/min wer