Dual-Doppler data collected from 1646 to 1648 MDT on 14 July, 1982 in Colorado were employed to study the eddy kinetic energy budget in the subcloud layer of a microburst-producing thunderstorm during its mature stage. Each term in the budget equation was computed from the Doppler-derived winds and retrieved thermodynamic fields within the 10 by 10 km horizontal domain. Results show that in the atmospheric boundary layer (ABL) where the microburst dominates, the turbulent flow extracts energy from the mean flow in order for the microburst to maintain its strong diverging outflow at low levels. The vertical transport of eddy kinetic energy is predominantly downward in the low layer due to the organized downdrafts in the microburst area. The horizontal flux convergence (divergence) of eddy kinetic energy by the mean and eddying motions is approximately balanced by that of the vertical flux divergence (convergence). Similarly, the contributions from the pressure and buoyancy production terms are nearly in balance. As a result, a net change of the eddy kinetic energy generation in the subcloud layer is relatively small in comparison with the individual term in the budget equation.