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The sub-nanomolar binding of DNA-RNA hybrids by the single-chain Fv fragment of antibody S9.6

✍ Scribed by Phillips, Damilola D.; Garboczi, David N.; Singh, Kavita; Hu, Zonglin; Leppla, Stephen H.; Leysath, Clinton E.


Book ID
120515597
Publisher
John Wiley and Sons
Year
2013
Tongue
English
Weight
331 KB
Volume
26
Category
Article
ISSN
0952-3499

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Cells from a normal donor, a B-CLL and a T-ALL were labeled with an FITC conjugated rabbit antihuman IgM and hybridized with a rhodamine-conjugated cloned DNA coding for the c mu segment. The cells were measured simultaneously by immunofluorimetry for their surface content of IgM and their amount of