The study of photoconduction of artificial lipid membranes incorporating rhodopsin. The simultaneous changes of membrane conduction and rhodopsin fluorescence

✍ Scribed by E. E. Fesenko; V. L. Ratner; A. L. Lyubarskiy

• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 264 KB
• Volume: 3
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00423232

✦ Synopsis

The protein fluorescence changes of rod outer segment fragments during bleaching were studied. Flash caused a fluorescence intensity drop by about 6%. The time constant of this process was• 30 msec and coincided with the time constant of increasing the permeability of an artificial lipid membrane containing rhodopsin and of Metarhodopsin I decay. In the presence of hydroxylamine the fluorescence intensity increases after the initial drop. The second process time constant was about 300 msec and coincided with the conductior~ drop time constant of the artificial membrane containing rhodopsin. A new intermediate -Metarhodopsin II1 is proposed, It has the Metarhodopsin II absorption spectrum, lives for about 300 msec at room temperature, does not react with hydroxylamine, and increases the permeability of a disk membrane.