The structure of the oligosaccharide moieties of pig lung glycolipids

✍ Scribed by E.P. Adams; G.M. Gray

• Publisher: Elsevier Science
• Year: 1967
• Tongue: English
• Weight: 405 KB
• Volume: 1
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(67)90014-x

✦ Synopsis

The chemical structures of the oligosaccharide moieties of four glycolipids isolated from pig lung were determined.

The identification of methylglycosides obtained by a two-stage methylation-methanolysis of each glycolipid fraction provided evidence for its structural characterisation. The methyl glycosides were analysed by gas chromatography. Evidence of fl-linkages was obtained from the infra-red spectra of the glycolipids.

The ceramide monohexoside was fl-glucosyl-(l~l)-ceramide. B-galactosyl-(l~4)-flglucosyl-(1 ~l)-ceramide was the major component (> 95 ~) of the ceramide dihexoside fraction. Small amounts of fl-galactosyl-(1 ~4)-fl-galactosyl-(1 ~l)-ceramide (2 ~) were present. The ceramide trihexoside and the ceramide hexosaminyltrihexoside fractions were identified as galactosyl-(l~4)-galactosyl-(l~4)-glucosyl-(1-~l)-ceramide and N-acetylgalactosaminyl-( 1 ~3)-galactosyl-(1 ~4)-galactosyl-(1 ~4)-glucosyl-(1 ~ 1) -ceramide respectively.