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The structure of the lipopolysaccharide O antigen from Yersinia ruckeri serotype 01

✍ Scribed by Linda M. Beynon; James C. Richards; Malcolm B. Perry

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
915 KB
Volume
256
Category
Article
ISSN
0008-6215

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✦ Synopsis

The 0 antigen obtained from the lipopolysaccharide of Yersinia mckeri serotype 01, by mild acid hydrolysis, is composed of a branched tetrasaccharide repeating unit containing 2-acetamidino-2,6-dideoxy-L-galactose (L-FucAm), 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), and 7-acetamido-3,5,7,9-tetradeoxy-5-(4-hydroxybutyramido)-o-glycero-L-galacnonulosonic acid (r_-Sug). Partial hydrolysis of the 0 antigen with 0.1 M HClafforded a trisaccharide and a tetrasaccharide having nonulosonic acid at their reducing ends. Cleavage of the 0 antigen with anhydrous methanolic hydrogen fluoride afforded the methyl glycoside derivatives of a trisaccharide and a tetrasaccharide. 'H and 13C NMR analysis, including 'H-13C heteronuclear multiple bond correlation spectroscopy to locate the N-acyl substituents, together with mass spectrometric analysis of the above oligosaccharides, allowed the structure of the O-specific polysaccharide to be assigned as: f, 3)+x-L-FucpAm-(1 -+ 3)-a-D-GlcpNAc-(1 + @-(u-L-Sug-(2+,, 4 t P-D-G:cpNAc

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