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The structure of the cysCDHIJ region in unstable cysteine or methionine requiring mutants of Salmonella typhimurium

โœ Scribed by Kingsman, Alan J.

Publisher
Springer
Year
1977
Tongue
English
Weight
532 KB
Volume
156
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

A genetic method was devised to test the hypothesis that in some cysteine or methionine requiring (cym) mutants of Salmonella typhimurium suppression of auxotrophy is due to an insertion at the site of the cym mutation. It was found that suppressed strains have an insertion of about 9kb in the cysCDHIJ region and that in unstable suppressed strains it is the instability of this insertion which results in the segregation of cym auxotrophs.

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Method of isolation of cysteine constitu
Method of isolation of cysteine constitutive mutants of the cysteine regulon in Salmonella typhimurium
โœ Sledziewska, E. ;Hulanicka, D. ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Springer ๐ŸŒ English โš– 404 KB

A method for selection of constitutive cysB mutation is described which takes advantage of the resistance of cysteine constitutive mutants to 1,2,4-triazole. Since cysM cysK double mutants are cysteine auxotrophs, by selecting for triazole resistance in cysM strains, mutants arising under this condi