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The Structure of Calcium-Free Human m-Calpain: Implications for Calcium Activation and Function

✍ Scribed by David Reverter; Hiroyuki Sorimachi; Wolfram Bode

Book ID
114341987
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
733 KB
Volume
11
Category
Article
ISSN
1050-1738

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Regulation of the mM-calcium-requiring form of calpain (m calpain) was studied in SH-SY-5Y human neuroblastoma cells. Immunoblot analysis demonstrated that the vast majority of m calpain is localized within cytosolic pools. Calpain activation was monitored as a function of autolysis within intact ce