## Abstract ## Objective To assess the effects of human intervertebral disc aggrecan on nerve growth and guidance, using in vitro techniques. ## Methods Aggrecan extracted from human lumbar intervertebral discs was incorporated into tissue culture substrata for the culture of the human neuronal
The structure and degradation of aggrecan in human intervertebral disc
β Scribed by Peter J. Roughley; Lee I. Melching; Terrence F. Heathfield; Richard H. Pearce; John S. Mort
- Publisher
- Springer
- Year
- 2006
- Tongue
- English
- Weight
- 210 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0940-6719
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## Abstract Human intervertebral disc when maintained in organ culture released a latent caseinβdegrading metalloproteinase into the medium in a manner analogous to cultures of human cartilage. This enzyme was demonstrated to be immunologically identical to prostromelysin. It was also found that th
Fifteen lumbar spines were collected postmortem. The interverte-bra1 discs were assigned morphological grades and were analyzed for water, collagen, and proteoglycan. In order of increasing degeneration, five grade 0, four grade 1,45 grade 2, 12 grade 3A, and nine grade 3B discs were identified. The