The structural and functional polarity of the hepatic human/rat hybrid WIF-B is a stable and dominant trait

✍ Scribed by Virginie Bender; Pilar Bravo; Catherine Decaens; Doris Cassio

• Book ID: 102851456
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 721 KB
• Volume: 30
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.510300436

✦ Synopsis

Methods

Materials. F-12 Coon' s modification culture medium, all medium supplements, and fluorescent phalloidin were obtained from Sigma Chemical Company (St. Louis, MO). Fetal bovine serum was from Multiser (Cytosystems, Castle Hill, Australia). Geneticin (G418 sulphate) was purchased from GIBCO LTD (Paisley, Scotland). Rabbit polyclonal and mouse monoclonal antibodies directed against several apical (dipeptidylpeptidase IV [DPPIV], HA4, 5Ј nucleotidase [5Ј-NT], polymeric IgA receptor [pIgA-R]) and basolateral (CE9, HA321) plasma membrane markers 6 were generous gifts from A. Hubbard (Johns Hopkins University, Baltimore, MD). Rabbit polyclonal antibodies directed against the apical alkaline phosphodiesterase B10 and against ezrin were kindly provided by M. Maurice 15 and M. Arpin, 16 respectively. Rat monoclonal antibody R40-76