The stromatolysis of sheep erythrocytes by Triton-X-100
β Scribed by D.A. Cooney; J.C. Drake
- Book ID
- 118875483
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 586 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0014-4827
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π SIMILAR VOLUMES
A simple permeabilization procedure has been developed which allows the reliable determination of enzyme activities in situ in a variety of different microorganisms. Permeabilization is obtained by freezing cell suspensions in the presence of a low concentration of the anionic detergent Triton X-100
Studies of the effect of triton X-100 on the fragility of rabbit erythrocytes and on shape and volume changes of rabbit and sheep erythrocytes suspended in an isotonic salt solution suggest that this subtance alters the erythrocytes of other species in a manner similar to that reported previously fo