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The stromatolysis of sheep erythrocytes by Triton-X-100

✍ Scribed by D.A. Cooney; J.C. Drake


Book ID
118875483
Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
586 KB
Volume
54
Category
Article
ISSN
0014-4827

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A simple permeabilization procedure has been developed which allows the reliable determination of enzyme activities in situ in a variety of different microorganisms. Permeabilization is obtained by freezing cell suspensions in the presence of a low concentration of the anionic detergent Triton X-100

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Studies of the effect of triton X-100 on the fragility of rabbit erythrocytes and on shape and volume changes of rabbit and sheep erythrocytes suspended in an isotonic salt solution suggest that this subtance alters the erythrocytes of other species in a manner similar to that reported previously fo