The stimulation of yeast growth and respiration by compounds produced by yeast cells irradiated with ultraviolet light

✍ Scribed by Adelstein, S. James ;Hershey, Falls B. ;Loofbourow, John R. ;Sizer, Irwin W.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1952
• Tongue: English
• Weight: 399 KB
• Volume: 40
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030400208

✦ Synopsis

FIVE FIGURES

The ability of products from ultraviolet damaged cells to stimulate the growth and proliferation of normal yeast suspensions has been studied in some detail (Loofbourow, '48). Fardon, Carroll and Ruddy ('37) established that the oxygen consumption of living yeast cells was stimulated by direct ultraviolet and x-radiation. They showed that the addition of ultraviolet killed cells to living cultures produced respiratory stimulation. Fardon and Ruddy ( '37) added centrifuged and filtered fluids from irradiated yeast to non-irradiated yeast suspensions. Respiratory stimulation by the cell-free fluid was pronounced, while that due to the washed cells was negligible.

Sizer and Loofbourow (see Loofbourow, '48), using centrifuged and filtered fluids from ultraviolet irradiated aqueous suspensions of S. cerevisiue, have increased both the endogenous and exogenous respiration of Baker's yeast. The effect has been blocked with cyanide. These preparations, tested for proliferation promoting effects on yeast cultures grown in