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The spliced variant of hepatitis B virus protein, HBSP, interacts with Bcl-2/Bcl-xl in vitro and induces apoptosis in HepG2 cells

✍ Scribed by Yi Wei Lu; Yu Dan Ren; Jing Bai; Wei Ning Chen

Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 293 KB
Volume: 60
Category: Article
ISSN: 1521-6543
DOI: 10.1002/iub.108

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✦ Synopsis

Abstract

We have recently reported that the naturally occurring spliced variant of Hepatitis B virus protein, HBSP, displayed proapoptotic activity through its BH3 domain. To investigate whether the BH3 domain in HBSP interacted with Bcl‐2 family of proteins, HBSP and Bcl‐2 family of proteins were cloned and expressed in our mammalian two‐hybrid system. Interaction assays were carried out in HepG2 cells and measured by the activity of the reporter gene product luciferase. Our results indicated that HBSP interacted with Bcl‐2/Bcl‐xl in vitro and induced apoptosis in HepG2 cells. © 2008 IUBMB IUBMB Life, 60(10): 700–702, 2008

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