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The specific assay of heparin by its chemical properties

✍ Scribed by Philip Band; Aaron Lukton

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
400 KB
Volume
120
Category
Article
ISSN
0003-2697

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✦ Synopsis

A sensitive and specific chemical assay for heparin is herein presented. It is based on heparin's ability to catalyze the acidic hydrolysis of the cationic dye Auramine 0. The assay can detect as little as 1 rg of heparin, and is specific in that it distinguishes heparin from all other glycosaminoglycans tested. The applicability of the assay and interference due to the presence of proteins, organic cations, and inorganic salts is discussed.

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A simple method to tritiate commercial samples of heparin is described. Heparin is reacted with NaB3H4 at pH 8.0 for 3 h at room temperature, after which the 3H-labeled mucopolysaccharide is isolated by gel filtration. Using NaB3& of low specific radioactivity (227-555 mCi/mmol), [3H]heparins contai