The soluble citric acid cycle enzymes ofDrosophila melanogaster. II. Tissue and intracellular distribution of aconitase and NADP-dependent isocitrate dehydrogenase

✍ Scribed by David J. Fox; Marianne Conscience-Egli; Erika Abächerli

• Publisher: Springer
• Year: 1972
• Tongue: English
• Weight: 660 KB
• Volume: 7
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00486086

✦ Synopsis

Aconitase (aconitate hydratase) (AH) and NADP-dependent isocitrate dehydrogenase (IDH-NADP) are found in every larval and adult Drosophila tissue. Their specific activities as well as the ratios of their absolute activities differ significantly from tissue to tissue. There are tissue-specific differences in the pattern of IDH-NADP isozymes in adults and in larvae. No clear-cut tissuespecific patterns exist for AH isozymes. Most of the activity of both enzymes is found in the supernatant fraction of whole fly homogenates. Only 35%o of the AH activity and 16°//oo of the IDH-NADP activity are associated with mitochondria. The patterns of supernatant and mitochondrial IDH-NADP isozymes are the same. On the other hand, the supernatant possesses AH isozymes not found in the mitoehondria.