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The single cell gel electrophoresis assay for induced DNA damage (comet assay): measurement of tail length and moment

✍ Scribed by Ashby, J.; Tinwell, H.; Lefevre, P.A.; Browne, M.A.


Book ID
121653953
Publisher
Oxford University Press
Year
1995
Tongue
English
Weight
363 KB
Volume
10
Category
Article
ISSN
0267-8357

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The single cell gel electrophoresis (SCGE) assay, or comet assay, is a highly sensitive method to measure DNA damage. The principle of the assay is based on the conversion of single-strand breaks and alkali-labile sites to double-strand breaks via alkaline lysis. The cells are subjected to electroph