The simultaneous production of both Hly- and Hpm-like hemolysins is characteristic of the Proteus penneri species
✍ Scribed by Dr. L. Serwecinska; M. Pytlos; S. Łukomski; A. Jaworski; A. Rózalski
- Book ID
- 102911683
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 799 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
Clinical isolates of Proteus penneri were tested for the presence of genes encoding hemolytic activity. Strains possessing DNA sequences similar to the __hly__CABD genes in Escherichia coli were found. Each secreted a 110 kDa protein which reacted with a specific anti‐__Hly__A antiserum. Southern blotting analysis revealed that the __Hin__dIII restriction fragment pattern for the __hly__CABD genes of these strains was conserved. Similarly, the chromosomal location of these genes is relatively conserved based on the pattern of NotI digested DNA fragments separated by pulsed field gel electrophoresis. One strain carried an additional copy of the __hly__CABD determinant which was mapped on a second NotI genomic fragment. All strains contained also chromosomally encoded sequences related to the __hpm__BA genes originally cloned from Proteus mirabilis. All strains produced a 166 kDa exoprotein detected in immunoblots with a specific antiserum raised against __Hpm__A hemolysin. The __hpm__BA genes were located on other NotI fragments than __hly__CABD genes. In contrast to the other Proteae, the simultaneus production of both hemolysins seems to be a common characteristics of Proteus penneri isolates.