✦ LIBER ✦

The simultaneous measurement of the electrophoretic mobility and diffusion coefficient in bovine serum albumin solutions by light scattering

✍ Scribed by B.R. Ware; W.H. Flygare

Publisher: Elsevier Science
Year: 1971
Tongue: English
Weight: 643 KB
Volume: 12
Category: Article
ISSN: 0009-2614
DOI: 10.1016/0009-2614(71)80621-8

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✦ Synopsis

A new technique for mea@% electrophoretic mobilities is described. The method employs a combination of ekctrophoresis techniques with laser beat frequency spectroscopy to measure the Doppler shift of the Raykkh scattered light from a solution of charged bovine serum albumin mokarles in an ekctric field. Equations are presented to desqibe the electric field effects, and the experiments demonstrate the simultaneous measurement of the .diffusion axfficient and the ckarophoretic mobility. It is suggested that this technique may &a be employed for the quantitative analysis of mi&res of charged maaomobcuks in solution.

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