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The simultaneous assay of riboflavin 5-phosphate sodium and other water-soluble vitamins in liquid multivitamin formulations by liquid chromatograph

✍ Scribed by F.L. Lam; A. Lowande


Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
527 KB
Volume
6
Category
Article
ISSN
0731-7085

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✦ Synopsis


A simple liquid chromatographic method for the simultaneous assay of niacinamide, pyridoxine, thiamine and riboflavin 5'-phosphate sodium in liquid multivitamin formulations is described. A novel aspect of the procedure is the conversion of the riboflavin 5'-phosphate sodium to riboflavin (vitamin B2) by reaction with alkaline phosphatase prior to quantitative HPLC. Excellent recovery, linearity and accuracy are obtained by the proposed method.