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The sigma-like product of sporulation gene spoIIAC of Bacillus subtilis is toxic to Escherichia coli

โœ Scribed by Yudkin, M. D.

Publisher
Springer
Year
1986
Tongue
English
Weight
272 KB
Volume
202
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

The amino-acid sequence deduced from the nucleotide sequence of the spoIIAC gene of Bacillus subtilis has been shown to be homologous to that of the sigma subunit of the Escherichia coli RNA polymerase (Errington et al. 1985). I now describe results that indicate that this gene can be cloned in E. coli only under conditions in which it is not expressed.

๐Ÿ“œ SIMILAR VOLUMES

Effect of precisely identified mutations
Effect of precisely identified mutations in the spoIIAC gene of Bacillus subtilis on the toxicity of the sigma-like gene product to Escherichia coli
โœ Yudkin, M. D. ;Harrison, Diane ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 249 KB

Yudkin (1986) has shown that the spoIIAC gene of Bacillus subtilis cannot be cloned in Escherichia coli in such an orientation that it is expressed. This toxicity of the gene product has been attributed to its close homology with the sigma subunit of the E. coli RNA polymerase. The effect of six ind

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Modification of central metabolic pathway in escherichia coli to reduce acetate accumulation by heterologous expression of the bacillus subtilis acetolactate synthase gene
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## Abstract A novel metabolic engineering technique involving the redirection ofcellular carbon fluxes was employed to reduce acetate production in an __Escherichia coli__ culture. Metabolic engineering was achieved by cloning __E. coli__ the gene for the __Bacillus subtilis__ acetolactate synthase