Separation of o-phthalaldehyde derivativ
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Enrique Martínez-Force; Tahía Benítez
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Article
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1991
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Springer-Verlag
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English
⚖ 354 KB
Derivatization of primary amino acids with orthophthalaldehyde and B-mercaptoethanol forms derivatives that can be detected by absorbance at 340 rim. These were separated by reverse-phase high performance liquid chromatography (HPLC). A step-or more complex gradient (acetonitrile/phosphate buffer gr