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The separation of o-phthalaldehyde derivatives of amino acids by reversed-phase chromatography on octylsilica columns

✍ Scribed by Harry W. Jarrett; Kevin D. Cooksy; Bernice Ellis; James M. Anderson


Book ID
107714270
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
767 KB
Volume
153
Category
Article
ISSN
0003-2697

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Separation of o-phthalaldehyde derivativ
✍ Enrique Martínez-Force; Tahía Benítez 📂 Article 📅 1991 🏛 Springer-Verlag 🌐 English ⚖ 354 KB

Derivatization of primary amino acids with orthophthalaldehyde and B-mercaptoethanol forms derivatives that can be detected by absorbance at 340 rim. These were separated by reverse-phase high performance liquid chromatography (HPLC). A step-or more complex gradient (acetonitrile/phosphate buffer gr