The Sensitivity of Experiments Which Use Gradient Pulses for Coherence-Pathway Selection
β Scribed by G. Kontaxis; J. Stonehouse; E.D. Laue; J. Keeler
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 652 KB
- Volume
- 111
- Category
- Article
- ISSN
- 1064-1858
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In this work we present a new pulse sequence for the measurement of long-range heteronuclear coupling constants in which the optimization of coherence selection by pulsed field gradients offers a net increase in sensitivity. This type of experiments is extremely valuable for conformational studies o
## Abstract Novel pulse sequences incorporating the double pulsed field gradient spinβecho technique are presented that have particular use in identifying macromolecular bound water. The use of these sequences is illustrated using ribonuclease T~1~. Five amide protons crossβrelaxing with bound wate