The Selective Incorporation of Alkenes into Proteins in Escherichia coli
β Scribed by Zhiwen Zhang; Lei Wang; Ansgar Brock; Peter G. Schultz
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 114 KB
- Volume
- 114
- Category
- Article
- ISSN
- 0044-8249
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Protein obtained from several strains of Escherichia coli grown in the presence of [3,3'-14C]cystine contained the radiolabel in nearly all the other amino acids, suggesting catabolism of cysteine to pyruvic acid. Utilization in amino acid synthesis of the pyruvate thus generated can be blocked by g
## Abstract __The unsaturated amino acid 2βaminoβ3βmethylβ4βpentenoic acid (__E__βIle) was prepared in the form of its (2__S__,3__S__),(2__R__,3__R__) and (2__S__,3__R__),(2__R__,3__S__) stereoisomeric pairs. The translational activities of__ SS__β__E__βIle and__ SR__β__E__βIle were assessed in an_