Three independent relB mutants were studied. During amino acid starvation they all accumulate RNA and produce an inhibitor of in vitro protein synthesis; after starvation growth is retarded for hours. The mutants differ in the degree to which the common phenotype is expressed, but the characteristic
The segregation of the SbcA and Rac phenotypes in an Escherichia coli recB − mutant
✍ Scribed by Lloyd, Robert G.
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 678 KB
- Volume
- 134
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A technique is presented by which mutations can be introduced into the Escherichia coli chromosome by gene replacement between the chromosome and a plasmid carrying the mutant gene. The segregational instability of plasmids in E. coli is used with high efficiency to isolate E. coli mutants. The meth
Mutants of Escherichia coli have been isolated containing streptomycin suppressors that exhibit novel phenotypes with respect to strA alleles. In one class of these mutants, the suppressor effect parallels a strong binding to the ribosome of an additional protein in at least stoichiometric amounts.
Two Tn10 insertions that are in the rac locus of the chromosome of Escherichia coli have been isolated and characterized. The insertions are located at min 29.7 and min 30.0. The insertions are stable when an F123 rac::Tn10 episome is transferred to an F- rac+ recipient, but they are lost at a high
Expression of the ompF and ompC genes coding for major outer membrane proteins is osmoregulated by solutes, such as sucrose and NaCl, in the growth medium. The OmpR protein, a positive regulator of these genes, is involved in the osmoregulation (Dairi et al. 1985; Nara et al. 1984). In the present w
When a recA strain of Escherichia coli is transformed with a multicopy plasmid, pKY1, carrying the phr gene of E. coli, its extreme ultraviolet sensitivity is decreased. Derivatives of pKY1 were prepared in which the phr gene was inactivated by inserting transposon Tn1000. None of the 20 phr- deriva