✦ LIBER ✦

The secretion and post translational modification of interferons fromSaccharomyces cerevisiae

✍ Scribed by J. R. Piggott; M. E. E. Watson; S. M. Doel; A. R. Goodey; B. L. A. Carter

Publisher: Springer-Verlag
Year: 1987
Tongue: English
Weight: 795 KB
Volume: 12
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00368057

No coin nor oath required. For personal study only.

✦ Synopsis

Studies with three interferon molecules, IFN-alpha 2, IFN-beta 1, and a "hybrid" interferon, IFNX-430 are described which illustrate that both the expression and secretion characteristics of heterologous proteins in yeast cells reflect properties of the proteins themselves. Recombinant DNA techniques have also been used to demonstrate that the efficient processing of mature heterologous proteins from the yeast alpha factor secretion leader can be affected by sequences on the carboxyl side of the initial cleavage site. Secretion studies with heterologous proteins in S. cerevisiae are aimed at maximising yield, the percentage of extracellular product and correct amino terminus sequence. The results presented here show that all three factors are susceptible to currently unpredictable properties of the foreign sequence. This situation, in turn, means that heterologous proteins can be used as tools in the biochemical dissection of the yeast secretion process.

📜 SIMILAR VOLUMES

Post-translational modification of prote

Post-translational modification of proteins and the discovery of new medicine

✍ Raj B Parekh; Christian Rohlff 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 602 KB

Post-translational modifications are fundamental to processes controlling behaviour, including cellular signaling, growth and transformation. As the molecular basis of protein modifications in normal and disease processes are becoming better defined, so new strategies for designing therapeutic entit

The role of post-translational modificat

The role of post-translational modifications for learning and memory formation

✍ Berta Sunyer; Weifei Diao; Gert Lubec 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 378 KB 👁 1 views

Structural heterogeneity, post-translati

Structural heterogeneity, post-translational modifications, and biological activities of SV-IV, a major protein secreted from the rat seminal vesicle epithelium

✍ Pasquale Ferranti; Gianfranco Mamone; Antonio Malorni; John Guardiola; Paola Sti 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 224 KB 👁 2 views

The primary structure of purified SV-IV, a major secretory protein synthesized by the rat seminal vesicle (SV) epithelium, was analysed by electrospray mass spectrometry (ES-MS). The protein was found to be highly heterogeneous. The various components were separated and identified by reversed phase

Application of mass spectrometry to the

Application of mass spectrometry to the identification and quantification of histone post-translational modifications

✍ Michael A. Freitas; Amy R. Sklenar; Mark R. Parthun 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 393 KB 👁 1 views

## Abstract The core histones are the primary protein component of chromatin, which is responsible for the packaging of eukaryotic DNA. The NH~2~‐terminal tail domains of the core histones are the sites of numerous post‐translational modifications that have been shown to play an important role in t

Analysis of post-translational modificat

Analysis of post-translational modification and characterization of the domain structure of dynamin A from Dictyostelium discoideum

✍ Andreas Schlosser; Boris Klockow; Dietmar J. Manstein; Wolf D. Lehmann 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 284 KB

## Abstract The post‐translational modifications of the 96 kDa protein dynamin A from __Dictyostelium discoideum__ were analyzed using Q‐TOF mass spectrometry. The accurate molecular mass of the intact protein revealed a covalent modification causing an additional mass of 42 Da. The modification co

Differences in the post-translational mo

Differences in the post-translational modification of proteins by polyamines between weakly and highly metastatic B16 melanoma cells

✍ Simone Beninati; Alberto Abbruzzese; Massimo Cardinali 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 French ⚖ 717 KB

The identification of (y-glutamy1)polyamines in proteolytic digest of proteins from the cytosolic and particulate fractions of B 16-F I 0 and B 16-F I OLr6 cell lines, originating from a spontaneous tumor in C57BL/6 mice, indicates that polyamines are incorporated into melanoma cell proteins by tran