The science of stem cells

✍ Scribed by Slack, Jonathan Michael Wyndham

• Publisher: Wiley
• Year: 2017
• Tongue: English
• Leaves: 269
• Category: Library

✦ Synopsis

"Introduces all of the essential cell biology and developmental biology background for the study of stem cells. This book gives you all the important information you need to become a stem cell scientist. It covers the characterization of cells, genetic techniques for modifying cells and organisms, tissue culture technology, transplantation immunology, properties of pluripotent and tissue specific stem cells and, in particular, the relevant aspects of mammalian developmental biology. It dispels many misconceptions about stem cells, especially that they can be miracle cells that can cure all ills. The book puts emphasis on stem cell behavior in its biological context and on how to study it. Throughout, the approach is simple, direct, and logical, and evidence is given to support conclusions. Stem cell biology has huge potential for advancing therapies for many distressing and recalcitrant diseases, and its potential will be realized most quickly when as many people as possible have a good grounding in the science of stem cells. Content focused on the basic science underpinning stem cell biology Covers techniques of studying cell properties and cell lineage in vivo and in vitro Explains the basics of embryonic development and cell differentiation, as well as the essential cell biology processes of signaling, gene expression, and cell division Includes instructor resources such as further reading and figures for downloading Offers an online supplement summarizing current clinical applications of stem cells Written by a prominent leader in the field, The Science of Stem Cells is an ideal course book for advanced undergraduates or graduate students studying stem cell biology, regenerative medicine, tissue engineering, and other topics of science and biology."-- �Read more...

Abstract:

Introduces all of the essential cell biology and developmental biology background for the study of stem cells This book gives you all the important information you need to become a stem cell scientist. �Read more...

✦ Table of Contents

Content: ""Title Page""
""Copyright Page""
""Contents""
""Preface""
""About the Companion website""
""Chapter 1 What is a Stem Cell?""
""Stem Cell Markers""
""Label-Retention ""
""The Niche""
""Asymmetric Division and Differentiated Progeny""
""Clonogenicity and Transplantation""
""In Vivo Lineage Labeling""
""Conclusions""
""Further Reading""
""Chapter 2 Characterizing Cells""
""Histological and Anatomical Methods""
""Histological Sections""
""Fixation""
""Sectioning""
""Staining""
""Electron Microscopy""
""Fluorescence Microscopy""
""Wholemounts""
""Immunostaining"" ""In Situ Hybridization""""Other Methods""
""RNAseq""
""Laser Capture Microdissection""
""Flow Cytometry""
""Dividing Cells""
""The Cell Cycle""
""Studying Cell Turnover""
""Reporters for the Cell Cycle""
""Identification of Very Slow Cell Turnover""
""Classification of Cell Types by Proliferative Behavior""
""Cell Death""
""Further Reading""
""Chapter 3 Genetic Modification and the Labeling of Cell Lineages""
""Introducing Genes to Cells""
""Transfection and Electroporation""
""Gene Delivery Viruses""
""Controlling Gene Expression""
""Tet System""
""Cre System"" ""Inhibiting Gene Activity""""CRISPRâ#x80
#x90
Cas9""
""Transgenic Mice""
""Animal Procedures""
""Modification of Embryonic Stem Cells""
""Types of Transgenic Mice""
""Cell Lineage""
""Cell Lineage, Fate Maps, Clonal Analysis""
""Use of CreER for Lineage Analysis""
""Retroviral Barcoding""
""Clonal Analysis in Humans""
""Further Reading""
""Chapter 4 Tissue Culture, Tissue Engineering and Grafting""
""Simple Tissue Culture""
""Media""
""Contamination""
""Growth in Culture""
""Cryopreservation and Banking""
""GMP Cultivation""
""Complex Tissue Culture"" ""Induced Differentiation""""Three Dimensional Cell Culture""
""Artificial Organs and Organoids""
""Grafting""
""The Immune System""
""T Cells""
""The Major Histocompatibility Complex""
""T and B Cell Responses""
""Reactions to a Graft""
""Immunosuppressive Drugs""
""Animal Experiments Involving Grafting""
""Further Reading""
""Chapter 5 Early Mouse and Human Development""
""Gametogenesis""
""Germ Cells""
""Mitosis and Meiosis""
""Primordial Germ Cells (PGCs)""
""Spermatogenesis""
""Oogenesis""
""Fertilization""
""Early Development""
""Preimplantation Phase"" ""Implantation Period â#x80
#x93
 Mouse""""Implantation Period â#x80
#x93
 Human""
""Ethical and Legal Issues Concerning the Early Human Conceptus""
""Sex Determination""
""X-Inactivation ""
""Imprinting""
""Cloning by Nuclear Transplantation (SCNT)""
""Further Reading""
""Chapter 6 Pluripotent Stem Cells""
""Mouse Pluripotent Stem Cells""
""Mouse Embryonic Stem Cells""
""Differentiation of Mouse ES cells""
""Mouse iPS Cells""
""Human Pluripotent Stem Cells""
""SCNT-Derived Embryonic Stem Cells ""
""Ethical Issues Concerning Human ES Cells""

✦ Subjects

Stem cells.;SCIENCE / Life Sciences / Cytology.;HEALTH & FITNESS / Diseases / General;MEDICAL / Clinical Medicine;MEDICAL / Diseases;MEDICAL / Evidence-Based Medicine;MEDICAL / Internal Medicine