The “rotary column” method for growth of large-scale quantities of cell monolayers

✍ Scribed by Giovanni G. Santero

• Publisher: John Wiley and Sons
• Year: 1972
• Tongue: English
• Weight: 938 KB
• Volume: 14
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260140506

✦ Synopsis

Abstract

A method and an apparatus that reduce the heavy expenses involved in traditional methods for the large‐scale monolayer production of primary and secondary, strain and line cells and of the biologicals derived therefrom are described. The method is based on the principle of gathering in a single unit a sheaf of columns by means of general manifolds fitted with cocks. The growth of cells on the glass walls is irrespective of the number, length, and diameter of the columns used. The apparatus, placed in a thermo‐adjustable room and connected to adequate devices which allow it to rotate on its longitudinal axis and to be set in a vertical position, need not be dismounted nor transported since it can be connected by a number of tubes to the necessary services. Sterilization is carried out by flowing steam and fluids are poured in or drained off by vacuum or pressure. A microscope fitted to the bearing structure allows the operator to observe the cell monolayers and the cytopathic effect of viruses on the whole length of the outer columns. During the various working stages pH is under continuous control and automatically adjusted. The whole working cycle is extensively described (cleaning, sterilization, seeding, incubation, trypsinization of the monolayer, culture and harvesting of the virus) and results compared with those obtained by traditional methods.